CA2 anticorps (Biotin)

N° du produit ABIN1607715

N° du produit (Fournisseur): 200-406-136-0100

Cet anticorps Lapin Polyclonal détecte spécifiquement CA2 dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour CA2 anticorps (Biotin) (ABIN1607715)

Antigène: CA2 (Carbonic Anhydrase II (CA2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CA2 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Détail du produit anti-CA2 anticorps (Biotin)

N° du produit (Fournisseur): 200-406-136-0100

Fournisseur: Rockland

Fonction: Carbonic Anhydrase II Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes].

Attributs du produit: Synonyms: rabbit anti-Carbonic Anhydrase II Antibody biotin Conjugation, biotin conjugated rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II

Purification: Anti-Carbonic Anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Carbonic Anhydrase II [Human Erythrocytes]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Carbonic Anhydrase II Biotin Antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Carbonic Anhydrase II in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur CA2

Antigène: CA2 (Carbonic Anhydrase II (CA2))

Autre désignation: CA2

Sujet: Background: Anti-Carbonic Anhydrase II antibody is necessary for bone resorption and osteoclast differentiation and is involved in the reversible hydration of carbon dioxide. It plays a role in the modulating of fluid secretion into the anterior chamber of the eye. Belonging to the alpha-carbonic anhydrase family, Anti-Carbonic Anhydrase II antibody is ideal for investigators involved in the areas of Cardiovascular and Cell Biology research.

ID gène: 760

NCBI Accession: NP_000058

UniProt: P00918